Screening,expression and characterization of nanobody targeting SARS-CoV-2 S proteins
10.13431/j.cnki.immunol.j.20240040
- VernacularTitle:抗SARS-CoV-2 S蛋白纳米抗体的筛选、表达和鉴定
- Author:
Dan JIANG
1
;
Weitong SHU
;
Guangqi LI
;
Huatao LAN
;
Qianying HUANG
;
Guangxian XU
Author Information
1. 523808 东莞,广东医科大学医学技术学院附属东莞第一医院东莞市分子免疫与细胞治疗重点实验室
- Keywords:
Nanobody;
SARS-CoV-2 S Protein;
purification and expression
- From:
Immunological Journal
2024;40(3):309-314
- CountryChina
- Language:Chinese
-
Abstract:
This study was performed to screen the sequence of specific nanobody targeting SARS-CoV-2 S protein,and to express and purify recombinant anti-SARS-CoV-2 nanobodies,and evaluate their characteristics and application potential.Based on the previously constructed natural bacteriophage nanobody display library,the anti-SARS-CoV-2 S protein nanobody sequences were screened,with biotinylated SARS-CoV-2 S protein receptor-binding domain antigen as target,by biotin-streptavidin liquid phase screening method,phage-ELISA and sequencing.Recombinant anti-SARS-CoV-2 S protein nanobody expression vectors were constructed,the protein was induced by IPTG and then purified,and their characteristics and application potential were analyzed by SDS-PAGE,Westem blot and ELISA methods.Two anti-SARS-CoV-2 S protein nanobody sequences were successfully obtained,and the corresponding prokaryotic expression bacteria were constructed.The recombinant anti-SARS-CoV-2 S protein nanobody was expressed and purified successfully,which can specifically bind to SARS-CoV-2 S protein.In conclusion,the recombinant anti-SARS-CoV-2 S protein nanobodies have screened and expressed successfully,which provides a basis for its application in SARS-CoV-2 detection and treatment,and facilitates related researches.
