Application of cell transfer technique in the diagnosis of fine needle aspiration cytology
10.3760/cma.j.cn112151-20210105-00019
- VernacularTitle:细胞转移技术在细针穿刺细胞学诊断中的应用
- Author:
Di CUI
1
;
Zhengxian CHEN
;
Longteng LIU
;
Dongge LIU
;
Lan CHEN
Author Information
1. 北京医院病理科 国家老年医学中心 中国医学科学院老年医学研究院 100730
- Keywords:
Cytological techniques;
Punctures;
Cell transfer technique;
Mount-Qnick gel
- From:
Chinese Journal of Pathology
2021;50(6):615-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the application of cell transfer technology to solve the problem of the limited number of fine needle aspiration cytology (FNAC) smears for various immunocytochemistry (ICC) staining and other auxiliary tests, and to enhance accurate cytological diagnosis.Methods:Thirty-four cases of FNAC smears from January 2020 to April 2020 in the Department of Pathology of Beijing Hospital were collected for investigation of the cell transfer technique. The materials in the most cell smear were divided and transferred to several glass slides. After de-staining, the recipient slides were stained with EnVision ICC. The technique was validated by comparing the consistency of the ICC of transferred cell smears and the corresponding immunohistochemical (IHC) staining on biopsies.Results:There were a total of 180 cell transfer slides from 34 cases, of which 174 had the same cell morphology, size and structure as the original smears, with the success rate of cell transfer of 96.7% (174/180). Totally 174 ICC stains were performed on the successfully transferred cell smears, of which 153 smears had available corresponding IHC staining of histologic specimens. Of these, 148 showed concordance between ICC staining and the IHC staining. Cells were successfully transferred in 96.7 % (148/153) of the cell sheets, keeping the same morphology and structure as compared to their original smears. The diagnosis of all 34 FNAC cases was the same to that of their corresponding pathology on biopsies with 100 % concordance.Conclusions:The cell transfer technique is a simple and effective way to make full use of diagnostic cells on a cell smear, and is valuable for accurate cytological diagnosis.
