Preparation and immune response characterization of self-assembled nanoparticle vaccine candidates for monkeypox
10.13431/j.cnki.immunol.j.20240000
- VernacularTitle:猴痘自组装纳米颗粒候选疫苗的制备及免疫应答特征研究
- Author:
Lingyun TAN
1
,
2
;
Hui WANG
;
Xiaolan YANG
;
Fangzhou WANG
;
Yan LI
;
Deyan LUO
;
Hongjing GU
Author Information
1. 230032 合肥,安徽医科大学基础医学院
2. 100071 北京,军事科学院军事医学研究院微生物流行病研究所病原微生物生物安全全国重点实验室
- Keywords:
Monkeypox antigen A29L;
Self-assembling nanoparticle vaccines;
Humoral immunity;
Cellular immunity
- From:
Immunological Journal
2024;40(1):96-103
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to prepare a self-assembled nanoparticle monkeypox vaccine candidate and study its immune response characteristics,so as to provide reference test data for its vaccine design.The antigen protein A29L-SpyTag and the backbone protein Mi3-SpyCatcher were expressed and purified by prokaryotic system,and nanoparticles A29L-Mi3 were prepared by chemical assembly,then the antibody titers were determined by ELISA,the antibody neutralization was determined by plaque test,and the cytokine secretion of lymphocytes was determined by flow cytometry to describe the immune response characteristics.Data showed that A29L-Mi3 nanoparticles were successfully prepared,and the particles were uniformly distributed in hollow cages,with an average particle size of(29±0.19)nm.After the A29L-Mi3 nanoparticle vaccine candidate was combined with SP01 adjuvant,the neutralizing antibody titer was stronger than that of the A29L protein candidate,and the A29L-Mi3 nanoparticle vaccine candidate could obtain neutralizing antibodies with similar titers after two immunizations.The level of mouse T lymphocyte immune response activated by the A29L-Mi3 nanoparticle vaccine candidate was higher than that of the A29L protein vaccine candidate.In conclusion,A29L-Mi3 protein nanoparticles with uniform structure have successfully assembled in vitro,which has strong immunogenicity and improved neutralization ability after combination with SP01 adjuvant,thus provided reference data for the optimization of immune programs.In addition,the level of cellular immune response is higher than that of A29L protein alone,which provides a reference for the design and development of monkeypox vaccine.
