Expression of PXMP4 in breast cancer tissues and its effects on biological behavior of breast cancer cells
10.13315/j.cnki.cjcep.2024.09.006
- VernacularTitle:乳腺癌组织中PXMP4的表达及其对乳腺癌细胞生物学行为的影响
- Author:
Wei LI
1
;
Jingyu ZHANG
;
Haixia BU
;
Qingqing CHEN
;
Xupeng SUN
;
Xinlai QIAN
;
Guoyang HE
Author Information
1. 新乡医学院法医学院,新乡 453003
- Keywords:
breast neoplasm;
PXMP4;
EMT;
proliferation;
in-vasion;
metastasis
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(9):923-930
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To detect the expression of peroxiso-mal membrane protein 4(PXMP4)in breast cancer tissues and to explore the effect of PXMP4 on the proliferation,invasion,and epithelial-mesenchymal transition(EMT)of breast cancer cells.Methods Bioinformatics and immunohistochemistry(IHC)were used to detect the expression of PXMP4 in breast cancer tissues.In breast cancer cells,Western blot was used to detect the expression of Cyclin D1,E-cadherin,vimentin and N-cadherin after knockdown and overexpression of PMXP4.The proliferation ability of breast cancer cells was analyzed by CCK-8 and plate cloning assay.Scratch healing and Transwell assay an-alyzed the migration and invasion ability of breast cancer cells.Lentivirus was used to construct a PXMP4-silenced MCF-7 cell line,and the PXMP4-silenced MCF-7 cells were injected into the subcutaneous or tail vein of mice to observe lung metastasis and the number of subcutaneous tumors.Results Bioinformat-ics and IHC showed that the expression of PXMP4 in breast cancer tissues was significantly increased(P＜0.05),and the prognosis of breast cancer patients with high expression of PXMP4 was poor(P＜0.05).The clinicopathological analysis showed that the expression of PXMP4 was correlated with tumor grade and lymph node metastasis(P＜0.05).In vitro knock-down of PMXP4 inhibited the proliferation,invasion and EMT process of breast cancer cells(P＜0.05).Conversely,overex-pression of PXMP4 promoted the proliferation,invasion and EMT process of breast cancer cells(P＜0.05).In vivo,the number of lung metastases,the size of subcutaneous tumor,and the expression of Ki67 in tumor tissue were significantly de-creased after silenced PXMP4(P＜0.05).Conclusion PXMP4 is related to tumor grading and lymph node metastasis.PXMP4 promotes proliferation,invasion and EMT process of breast cancer cells.
