Expression of autophagy-related protein ATG7 in breast cancer and its clinical significance
10.13315/j.cnki.cjcep.2024.09.005
- VernacularTitle:自噬相关蛋白ATG7在乳腺癌中的表达及其临床病理意义
- Author:
Yan QU
1
;
Tingting SHI
;
Juanru WANG
;
Jun LUO
;
Zhihao WANG
;
Wenwu LUO
;
Qianying GUO
;
Min ZHAO
;
Zhengsheng WU
Author Information
1. 安徽医科大学基础医学院病理学教研室,合肥 230032
- Keywords:
breast neoplasm;
autophagy;
ATG7;
proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(9):917-922
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the expression of autoph-agy-related 7(ATG7)in breast cancer and its effect on the breast cancer development.Methods Immunohistochemistry(IHC)was used to detect ATG7 protein expression in breast cancer tissues and the relationship between ATG7 and clinico-pathological features was analyzed.ShRNA was used to interfere with the expression of ATG7 in breast cancer cell line MCF-7.Puromycin was used to screen for stably transfected cells and Western blot was used to detect transfection efficiency.The effect of ATG7 knockdown cells on proliferation ability was de-tected by CCK8 and clone formation experiments.The effect of ATG7 knockdown cells on tumorigenicity in vivo was detected by subcutaneous tumor formation experiment in nude mice.Results IHC showed that ATG7 expression in breast cancer tissues was mainly localized in cytoplasm,and its expression was significant-ly correlated with tumor size and Ki67 expression(P＜0.05).ATG7-shRNA significantly interfered with ATG7 expression in breast cancer cells MCF-7.CCK8 and clone formation experi-ments showed that ATG7 knockdown promoted the cell prolifera-tion compared with the control group.The experiment of subcu-taneous tumor formation in nude mice showed that the tumor for-mation ability of mice was significantly increased after ATG7 knockdown compared with the control group.Conclusion ATG7 may inhibit the proliferation capacity of breast cancer and could be a potential target for breast cancer therapy.
