Expression and clinical significance of Hsp90α in colon adenocarcinoma
10.13315/j.cnki.cjcep.2024.08.013
- VernacularTitle:结肠癌中Hsp90α的表达及临床意义
- Author:
Yang ZHANG
1
,
2
;
Xiaoting WU
;
Fang WANG
;
Xingchen DAI
;
Yuhuan ZHANG
;
Yujing GAO
Author Information
1. 宁夏医科大学第一临床医学院,银川 750004
2. 宁夏医科大学总医院消化内科,银川 750004
- Keywords:
colon neoplasms;
Hsp90α;
immune infiltration;
immunohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(8):845-852
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression and po-tential clinical value of heat shock protein 90α(Hsp90α)in co-lon adenocarcinoma.Methods The expression level of Hsp90αin colon adenocarcinoma and its relationship with clinicopatho-logical features,prognosis and immune cell infiltration were ana-lyzed by bioinformatics and immunohistochemistry.The prolifer-ation ability of colon cancer cells before and after Hsp90AA1 knockout was detected by CCK-8 cell proliferation assay and plate cloning assay.Results The bioinformatics tools showed that Hsp90AA1 was abnormally higher in colon cancer tissues than adjacent tissues,and the higher the expression level,the worse the prognosis of patients.The expression of Hsp90AA1 was positively correlated with the infiltration levels of CD4+T cells(Th2),CD8+T cells,myeloid inhibitory cells,Tregs cells,neutrophils,macrophages,M1 macrophages and M2 macropha-ges.Immunohistochemical results showed that the expression of Hsp90α in colon cancer tissues was significantly higher than in its adjacent tissues.The expression of Hsp90α was related to age(P＜0.05),not related to gender,tumor size,location,clini-cal classification,differentiation degree,tumor node metastasis,lympho-vascular invasion,perineural invasion(P＞0.05).The high expression of Hsp90α was an independent risk factor for the prognosis of colon cancer patients.The results of cell experi-ments showed that Hsp90AA1 knockout inhibited the growth and proliferation of colon cancer cells.Conclusion Hsp90α is highly expressed in colon adenocarcinoma,which may be a po-tential molecular marker for poor prognosis of colon adenocarci-noma.
