Pathological characteristics of heart and spleen in a kawasaki disease model induced by candida albicans watersoluble fraction
10.13315/j.cnki.cjcep.2024.07.011
- VernacularTitle:白色念珠菌水溶物诱导的川崎病模型心脏与脾脏病理学特征
- Author:
Jiaxin ZHANG
1
;
Zongli ZHANG
;
Yifan LUO
;
Tao LI
;
Shibing XI
Author Information
1. 湖北医药学院附属太和医院儿科,十堰 442000
- Keywords:
Kawasaki disease;
candida albicans watersoluble fraction;
splenomegaly;
macrophage infiltration
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(7):719-724
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigating the pathological characteristics of the heart and spleen in a Kawasaki disease(KD)model induced by candida albicans watersoluble fraction(CAWS)and to provide a research basis for the pathological mechanisms of KD.Methods Intraperitoneal injection of CAWS was used to establish a KD model in C57BL/6 mice.Specimens of the heart,aorta,and spleen were collected to measure body weight and the weights of the heart and spleen.HE staining was utilized to examine the morphological alterations in the heart,aorta,and spleen.The expression levels of the ma-ture macrophage marker(F4/80),TNF-α,and IL-1β in the spleen were measured using qRT-PCR and immunofluorescence.its clinical pathological characteristics were analyze and relevant literatures were reviewed.Results Compared to the control group,there were no significant differences in the heart and body mass of the model group mice,but their spleen mass signif-icantly increased(P＜0.05).In the myocardial interstitium of model group mice,there was infiltration of mononuclear cells,disorder of the elastic fibers in the aortic wall along with mucoid degeneration,disruption of the splenic red and white pulp struc-ture,and significant macrophage infiltration.In the model group mice,the mRNA levels and immunofluorescence staining inten-sity of spleen F4/80(2.58-fold,P＜0.000 1),TNF-α(1.43-fold,P＜0.001),and IL-1β(3.62-fold,P＜0.000 1)were significantly higher than those in the control group.Conclusion Mice with KD models induced by CAWS can exhibit patholog-ical alterations akin to KD in the heart and aorta,significant en-largement of the spleen,infiltration by macrophages,with ele-vated expressions of TNF-α and IL-1β.
