Expression of C1GALT1 genes in gastric cancer and its effect on the biological behavior of BGC-823 cells in gastric cancer
10.13315/j.cnki.cjcep.2024.06.009
- VernacularTitle:C1GALT1基因在胃癌组织中的表达及其对胃癌BGC-823细胞生物学行为的影响
- Author:
Muchuan QIAO
1
;
Junru LI
;
Ling LUO
;
Tong XIA
;
Yanhua CHEN
;
Menglong HU
;
Hailong XIE
Author Information
1. 南华大学衡阳医学院肿瘤研究所/湖南省肿瘤细胞分子病理学重点实验室,衡阳 421009
- Keywords:
gastric neoplasms;
C1GALT1;
invasion;
migra-tion;
epithelial-mesenchymal transition
- From:
Chinese Journal of Clinical and Experimental Pathology
2024;40(6):603-608
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of C1GALT1 in gastric cancer and its effect on the biological be-havior of BGC-823 in gastric cancer cells.Methods The ex-pression of C1GALT1 mRNA and protein in gastric cancer tis-sues and normal gastric mucosa,gastric cancer cells and normal gastric mucosal cells was analyzed by bioinformatics,qRT-PCR and Western blot;the transient transfection of siRNA into BGC-823 cells was designed with C1GALT1 cDNA sequence as the target.Transwell assay was used to detect the effect of C1GALT1-siRNA on the migration and invasion ability of BGC-823 cells in gastric cancer.Western blot method detected the expression of epithelial-mesenchymal transition(EMT)-related proteins in BGC-823 after transfection of C1GALT1-siRNA.Re-sults C1GALT1 was highly expressed in gastric cancer tissues and cell lines BGC-823,SGC-7901 and MGC-803,and the ex-pression levels were positively correlated with gastric cancer pathological stages Ⅰ and Ⅱ(P＜0.05).After interfering with C1GALT1 in BGC-823 cells,the ability of migration and inva-sion decreased(P＜0.05),epithelial cell markers E-cadherin and Claudin-1 protein expression increased,while mesenchymal cell markers vimentin and Slug protein expression decreased(P＜0.05).Conclusion C1GALT1 is highly expressed in gastric cancer tissues and cells,silencing of C1GALT1 can inhibit mi-gration and invasion ability of gastric cancer,the mechanism may be related to EMT.
