Phenotypic Characterization of Ligamentum Flavum Cells from Patients with Ossification of Ligamentum Flavum.
10.3349/ymj.2009.50.3.375
- Author:
Zhao Ming ZHONG
1
;
Jian Ting CHEN
Author Information
1. Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. chenjt99@tom.com
- Publication Type:Original Article
- Keywords:
Cell phenotype;
ossification;
ligamentum flavum
- MeSH:
Adolescent;
Adult;
Aged;
Cells, Cultured;
Collagen Type I/genetics/metabolism;
Collagen Type II/genetics/metabolism;
Collagen Type VI/genetics/metabolism;
Female;
Humans;
Ligamentum Flavum/metabolism/*pathology;
Male;
Microscopy, Fluorescence;
Middle Aged;
Ossification, Heterotopic/metabolism/*pathology;
Osteocalcin/genetics/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Young Adult
- From:Yonsei Medical Journal
2009;50(3):375-379
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The objective of this study was to determine the phenotypic characterization of ligamentum flavum cells from patients with ossification of the ligamentum flavum (OLF). MATERIALS AND METHODS: Ligamentum flavum tissues were harvested from OLF and non-OLF patients during surgery. OLF and non-OLF cells were isolated from explant cultures. Cultured cells were analyzed using immunofluorescence staining and reverse transcription-polymerase chain reaction. RESULTS: OLF cells exhibited various appearances compared with the typical fibroblast-like morphology of non-OLF cells. Expressions of collagen type I and collagen type III were observed in OLF and non-OLF cells. OLF cells uniquely expressed osteocalcin, which is a marker for osteoblasts, and collagen type II which is a marker for chondrocytes, whereas they were negative in non-OLF cells. CONCLUSION: These findings indicate that OLF cells have phenotypic characterization of osteoblasts and chondrocytes which could play a role in the pathophysiology of OLF.
