Preventive Effects of Oligomerized Polyphenol on Estradiol-Induced Prostatitis in Rats.
10.3349/ymj.2009.50.3.391
- Author:
Dong Suk KIM
1
;
Eun Jin LEE
;
Kang Su CHO
;
So Jung YOON
;
Young Hoon LEE
;
Sung Joon HONG
Author Information
1. Department of Urology, Urological Science Institute, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. sjhong346@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Nonbacterial prostatitis;
inflammation;
polyphenol;
oligonol;
oxidative stress
- MeSH:
Animals;
Blotting, Western;
Body Weight/drug effects;
Estradiol/*adverse effects;
Flavonoids/*therapeutic use;
Immunoassay;
Male;
Phenols/*therapeutic use;
Prostate/drug effects/pathology;
Prostatitis/*chemically induced/metabolism/*prevention & control;
Rats;
Rats, Wistar;
Superoxide Dismutase/metabolism;
Tumor Necrosis Factor-alpha/metabolism
- From:Yonsei Medical Journal
2009;50(3):391-398
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS, NIH category III) accounts for 90-95% of prostatitis cases. However, standard treatment has not yet been established. It is known that polyphenols have an inhibitory effect on inflammation by their antioxidative capacity, and oligonol, a polyphenol derivative, has much higher bioavailability and bioactivity than common polyphenols. We investigated the anti-inflammatory effects and mechanisms of oligonol in estradiol-induced prostatitis rat models. MATERIALS AND METHODS: Prostatitis was induced by 17 beta-estradiol (E2) and dihydrotestosterone (DHT) in Wistar male rats (n = 20). Ten rats were placed in the oligonol-treated group and 10 in the E2 + DHT-treated group. The other 10 rats were also included as normal control group. Oligonol (60 mg/kg/day) was administered via gavage tube for 4 weeks. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and tumor necrosis factor-alpha (TNF-alpha) were quantified, and phosphorylation of IkappaBa and histological changes were also evaluated in prostatic tissue. RESULTS: The SOD and GPx activity showed tendencies to increase in the oligonol-treated group compared to the normal control group. TNF-alpha expression was slightly reduced in the oligonol-treated group. Western blotting demonstrated that phosphorylation of IkappaBa in the oligonol-treated group was significantly lower than in the normal control group. The E2 + DHT-treated group revealed severe atrophy of acinar epithelial cells and infiltration of leukocytes and lymphocytes in the prostate, however, the oligonol-treated group showed overall reduction in inflammatory features. CONCLUSION: This study demonstrates that oligonol improves estradiol-induced non-bacterial prostatitis by regulating phosphorylation of IkappaBa. These findings suggest that oligonol has a beneficial effect on prevention and treatment of CP/CPPS.
