Pathological significance of NY?ESO?1 expression in the diagnosis of myxoid liposarcoma
10.3760/j.issn.0529-5807.2019.03.013
- VernacularTitle:纽约食管鳞状上皮癌抗原1在黏液样脂肪肉瘤中的表达及病理诊断价值
- Author:
Shumin HEI
1
;
Hongjun WEI
;
Hua CHEN
;
Jigang WANG
Author Information
1. 青岛大学基础医学院病理学系266071
- Keywords:
Liposarcoma,myxoid;
Immunohistochemistry;
Reverse transcriptase polymerase chain reaction;
In situ hybridization,fluorescence;
NY?ESO?1 protein
- From:
Chinese Journal of Pathology
2019;48(3):225-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of New York esophageal squamous cell carcinoma antigen 1 (NY?ESO?1) in common types of mesenchymal myxoid tumors, and to investigate its significance in the diagnosis and differential diagnosis of myxoid liposarcoma. Methods A total of 43 formalin?fixed paraffin?embedded samples of mesenchymal myxoid tumors from the Affiliated Hospital of Qingdao University and Qingdao Municipal Hospital ranging between 2010 and 2017 were selected. NY?ESO?1 expression was detected by immunohistochemical staining. DDIT3 gene status was detected by fluorescence in situ hybridization (FISH). NY?ESO?1 mRNA was detected by reverse transcription?PCR (RT?PCR). Results Histopathology and FISH results confirmed that there were 11 cases of myxoid liposarcoma and 32 other types (including 7 cases of well?differentiated liposarcoma, 1 dedifferentiated liposarcoma, 3 lipomas, 2 lipoblastomas and 19 non?adipocytic tumors). Immunohistochemical staining showed that the positive expression propotion of NY?ESO?1 in myxoid liposarcoma was 11/11, and the positive location was the cytoplasm and nucleus of lipoblast cells. The expression intensity is higher in regions with round cell differentiation. Among the 32 cases of other mesenchymal myxoid tumors, only one well?differentiated liposarcoma showed positive immunoreactivity for NY?ESO?1. RT?PCR confirmed that 7 cases of myxoid liposarcoma (7/11) and one well?differentiated liposarcoma (1/7) had NY?ESO?1 mRNA expression. Conclusions NY?ESO?1 is positively expressed in myxoid liposarcoma. It can be served as a useful marker for the diagnosis and differential diagnosis of myxoid liposarcoma.
