Effects of miR-93 on proliferation and apoptosis of osteosarcoma cells
10.3760/cma.j.issn.0529-5807.2016.12.010
- VernacularTitle:miR－93对骨肉瘤细胞株增殖和凋亡的影响
- Author:
Ruijin WANG
1
;
Kourong SHI
;
Jun ZHANG
;
Jing ZHANG
;
Rongrong GAO
;
Shichang ZHU
Author Information
1. 同济大学医学院人体解剖与组织胚胎学系
- Keywords:
Osteosarcoma;
Cell proliferation;
Blotting,Western
- From:
Chinese Journal of Pathology
2016;45(12):866-870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-93 on proliferation and apoptosis of osteosarcoma cells and the possible mechanism .Methods The expression levels of miR-93 and the naked cuticle homolog 2 (NKD2) in 6 osteosarcoma cell lines (143B, HuO9, Saos2, MG63, U2OS and G292) and one osteoblast cell line hFOB 1.19 were determined by quantitative real-time PCR and Western blot assays, respectively. MiR-93 down-regulated 143B and HuO9 cells were constructed by lipofection transfection, and their proliferation and apoptosis were detected by MTT and flow cytometry assays , respectively.Luciferase reporter assay was used to determine whether the 3′UTR of NKD2 mRNA was a binding target of miR-93.In addition, 143B cells were transfected with NKD2 cDNA, and the effects of NKD2 on proliferation and apoptosis of osteosarcoma cells were investigated .Results Up-regulation of miR-93 and down-regulation of NKD2 were detected in osteosarcoma cell lines .MTT and flow cytometry assays showed that miR-93 promoted proliferation and inhibited apoptosis in osteosarcoma cells .Luciferase assay confirmed that miR-93 targeted NKD2 directly. In addition, overexpression of NKD2 inhibited proliferation and promoted apoptosis of osteosarcoma cells were found .Conclusions MiR-93 targets NKD2 to promote proliferation and inhibit apoptosis of osteosarcoma cells . The findings may have significant implications in the diagnosis and treatment of osteosarcoma .
