Application of rabbit monoclonal antibody GCET2 in diagnosis of diffuse large B cell lymphoma
10.3760/cma.j.issn.0529-5807.2016.12.006
- VernacularTitle:GCET2兔单克隆抗体的制备及其在弥漫性大B细胞淋巴瘤中初步应用
- Author:
Hongyang PAN
1
;
Xinxia ZHANG
;
Yuanyuan WENG
;
Ren ZHOU
Author Information
1. 宜康 杭州 生物技术有限公司
- Keywords:
Lymphoma,large B-Cell,diffuse;
Germinal center;
Antibodies,monoclonal
- From:
Chinese Journal of Pathology
2016;45(12):844-849
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a rabbit monoclonal antibody GCET 2 and to investigate its diagnostic value in the workup of diffuse large B-cell lymphoma ( DLBCL ) .Methods GCET2 rabbit monoclonal antibody was developed by using RabMAb ? technology , and its specificity was confirmed by ELISA, Western blot, immunohistochemistry ( IHC) and flow cytometry.A panel of immunomarkers including GCET2, CD10, bcl-6, MUM1, GCET1, FOXP1, Ki-67 and CMYC was evaluated in 81 cases of DLBCLs, 5 cases of follicular lymphomas ( FL) and 2 cases of Burkitt′s lymphomas.Results Rabbit monoclonal GCET2 antibody ( clone EP316 ) was developed with specificity for normal germinal center B-cells ( GCB ) and GCB origined lymphomas .In 81 cases of DLBCL , the positive rate of GCET2 was 43.2%( 35/81 ) , which was significantly higher than that of other germinal center markers .Moreover , among 81 DLBCLs, the proportions of high CMYC expression in GCET 2 negative and positive groups were 15.2%(7/46) and 2.8%(1/35), respectively.Conclusion GCET2 is a sensitive immunohistochemical marker for GCB derived lymphomas . Combined with other biomarkers , it may improve the diagnostic sensitivity of GCB-DLBCL.
