Alterations of mTOR pathway and autophagy in early type 2 diabetic cardiomyopathy in rats
10.3760/cma.j.issn.0529-5807.2016.10.008
- VernacularTitle:2型糖尿病大鼠早期心肌病 mTOR 信号通路的改变与自噬损伤
- Author:
Xiaohui SUN
1
,
2
;
Maoshan YIN
;
Yanling MU
Author Information
1. 250200 济南大学 山东省医学科学院医学与生命科学学院
2. 山东省医学科学院药物研究所
- Keywords:
Diabetes mellitus,type 2;
Diabetic cardiomyopathies;
Autophagy;
mTOR signaling pathway
- From:
Chinese Journal of Pathology
2016;45(10):707-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alterations of mTOR signaling pathway and autophagy in the development of type 2 diabetes and early diabetic cardiomyopathy and to study their roles in pathogenesis of diabetic myocardium.Methods A type 2 diabetes rat model was established by injection of streptozocin after five-week of high fat diet.The rats were randomly divided into control group, experiment group of 2 weeks and experiment group of 4 weeks.Alterations of mTOR, p-mTOR, S6K1, Beclin-1 and LC3-Ⅱexpression in myocardium were determined by Western blot and immunohistochemistry.Results Compared with the control group, the expression of mTOR, p-mTOR, S6K1, Beclin-1 and LC3-Ⅱ level increased significantly in the experiment group of 2 weeks.The expression of p-mTOR and S6K1 increased significantly in the experiment group of 4 weeks compared with those of the experiment group of 2 weeks.Conclusions mTOR signaling pathway is activated in early diabetic myocardial injury via autophagy.The findings may provide a new therapeutic target for diabetic cardiomyopathy.
