Significance of C-myc expression in T-lymphoblastic lymphoma/leukemia and its relation with prognosis
10.3760/cma.j.issn.0529-5807.2015.08.006
- VernacularTitle:C-myc与T淋巴母细胞淋巴瘤／白血病预后的相关性
- Author:
Yanhua ZHANG
1
;
Jing LI
;
Yanfeng XI
;
Wenqi BAI
;
Wei BAI
;
Ruifang SUN
Author Information
1. 山西医科大学研究生院
- Keywords:
Leukemia-lymphoma,adult T-cell;
Gene,myc;
Prognosis
- From:
Chinese Journal of Pathology
2015;(8):571-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the C-myc gene and protein in T lymphoblastic lymphoma/leukemia ( T-LBL/ALL) and its relationship to prognosis.Methods 60 cases of T-LBL/ALL with follow-up data were studied by using immunohistochemical EnVision method for CD 1a, CD3,εCD3, CD7, CD10, CD34, CD43,CD45RO,CD99,TDT,CD20,CD23,MPO,Ki-67 and C-myc.20 cases of reactive lymph nodes were selected as normal control group of C-myc gene and protein.Fluorescence in-situ hybridization ( FISH) for C-myc gene ( located on chromosome8q24 ) was performed to detect its breakage and gain.Results Among the 60 cases of T-LBL/ALL, immunohistochemistry results showed:the percentages of tumor cells expression of CD1a, CD3, εCD3, CD7, CD10, CD34, CD43, CD45RO, CD99and TDT were 38.3%(23/60), 75.0%(45/60), 45.0%(27/60),95.0%(57/60),36.7%(22/60),23.3%(14/60), 60.0%(36/60),41.7%(25/60),96.7%(58/60)and 93.3%(56/60).Separately, while CD20 ,CD23 and MPO were all negative.A figure of Ki-67 expression≤80% was found in 36 cases and >80% was found in 24 cases.The positive rate of C-myc protein was 66.7%(40/60) in 60 cases of T-LBL/ALL,was 0%(0/20) in 20 cases of reactivated lymphoid tissue (χ2 =26.67,P<0.05).C-myc protein expression was positively correlated with the mediatinal width and Ki-67 index ( P <0.05 ).Fluorescence in-situ hybridization results showed that among the 60 cases of T-LBL/ALL, C-myc gene with breakage of 8q24 was detected in 6 cases ( 10.0%) , and gains in 11 cases ( 18.3%).20 cases of reactive lymph nodes were not occurred breakage and gains of C-myc gene .It is not significant between C-myc gene and protein expression (P>0.05).In addition, in 60 cases of T-LBL/ALL, 12(20.0%) cases of C-myc protein and genetic abnormalities coexist.Log-rank analysis results: The prognosis of C-myc protein positive group was worse than negative group ( P<0.05 ).The relationship of C-myc gene and prognosis was not significant ( P>0.05).C-myc protein and genetic abnormality coexist is related with worse prognosis ( P<0.05 ).COX analysis results show that the C-myc protein positive group may be a independent poor prognosis factors ( P<0.05).Conclusions C-myc may play an important role on the development of T-LBL/ALL.It may be a independent prognosis factors .
