Expression of microRNA in ALK-negative anaplastic large cell lymphoma and CD30-positive peripheral T cell lymphoma, not otherwise specified
10.3760/cma.j.issn.0529-5807.2015.08.005
- VernacularTitle:ALK 阴性间变性大细胞淋巴瘤与 CD30阳性非特殊型外周T细胞淋巴瘤的microRNA 表达
- Author:
Chen WANG
1
;
Xiaoyan CHEN
;
Xin CHEN
;
Yihui HE
;
Liyu CAO
Author Information
1. 福建省立医院病理科
- Keywords:
Lymphoma,T cell;
MicroRNAs;
Polymerase chain reaction
- From:
Chinese Journal of Pathology
2015;(8):565-570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of microRNAs ( miRNAs ) in ALK-negative anaplastic large cell lymphoma and CD 30 positive peripheral T cell lymphoma ( not otherwise specified ) , and discuss the pathogenesis of miRNAs in ALK-negative anaplastic large cell lymphoma.Methods Three cases of ALK-negative anaplastic large cell lymphoma of lymph node , 3 cases of CD30-positive peripheral T cell lymphoma ( not otherwise specified ) of lymph node and 3 cases of reactive hyperplasia of lymph node were detected by high flow microarray of miRNAs.The method of real-time quantitative polymerase chain reaction was further applied for 7 miRNAs in 15 cases of ALK-negatie anaplastic large cell lymphomas of lymph node and 15 cases of CD30-positive peripheral T cell lymphoma ( not otherwise specified ) of lymph node.Results The significant difference of 13 miRNAs was found between ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma ( not otherwise specified ) ( P<0.05 ) , of which the result of 5 miRNAs was consistent with miRNAs expression spectrum:miR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p, the difference was statistically significant (P<0.05).Compared with reactive hyperplasia of lymph nodes, miR-664b-5p, miR-1275 and miR-4739 were significantly under-expressed (P=0.004, P=0.021, P=0.031) and miR-4736 and miR-504-5p were significantly over-expressed ( P=0.009, P=0.007) in ALK negative anaplastic large cell lymphoma.Conclusions MiR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p may become an important indicator in the differentiation ALK-negative anaplastic large cell lymphoma from CD 30-positive peripheral T cell lymphoma ( not otherwise specified).MiR-4739, miR-4736 and miR-1275 may play important role in pathogenesis of negative-anaplastic large cell lymphoma by target genes:TNFRSF8 and TMOD1.
