Mechanism of serotonin-promoted synthesis of osteoblast type Ⅰ collagen
10.3760/cma.j.issn.0529-5807.2015.07.019
- VernacularTitle:血清素促进成骨细胞Ⅰ型胶原蛋白的合成
- Author:
Yujing CAO
1
;
Cuitian LYU
Author Information
1. 河南省中医院创伤骨科
- Keywords:
Serotonin;
Collagen typeⅠ;
Smad2 protein;
Smad3 protein;
Lysyl oxidase
- From:
Chinese Journal of Pathology
2015;(7):509-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of serotonin-promoted osteoblast differentiation.Methods Expression levels of collagen Ⅰand lysyl oxidase ( LOX) in osteoblast were measured by RT-PCR after treated by (50, 100, 200 and 400 ng/L) serotonin.LOX siRNA effect was measured by Western blot, and protein levels of collagen Ⅰ were determined by ELISA after treated by serotonin.Expression levels of Smad2 and Smad3 in osteoblasts were also measured by RT-PCR after treated by serotonin.Moreover , expression levels of LOX were measured by RT-PCR after Smad3 was knockout.Results Serotonin promoted collagenⅠ and LOX expression.The expression level of collagenⅠ was significantly decreased by LOX siRNA.Furthermore , serotonin up-regulated the expression of Smad 2 and Smad3 in osteoblasts , and the expression level of LOX was inhibited by Smad 3 siRNA.Conclusion Serotonin promoted collagenⅠexpression by activating Smads signaling pathway and up-regulating the LOX expression.
