Long non-coding RNA Gm15577 is involved in mouse cerebellar neurogenesis
10.3760/cma.j.issn.0529-5807.2015.07.018
- VernacularTitle:长链非编码 RNA Gm15577参与小鼠小脑神经元增殖与分化
- Author:
Yongsong YUE
1
;
Weilong ZHANG
;
Chunying LIU
;
Yamei NIU
;
Weimin TONG
Author Information
1. 100005,中国医学科学院基础医学研究所 神经科学中心 北京协和医学院基础学院病理学系
- Keywords:
Cerebellum;
Nbs1;
Cell proliferation;
Cell differentiation;
Long non-coding RNA
- From:
Chinese Journal of Pathology
2015;(7):504-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify novel lncRNAs involved in cerebellar neurogenesis using neuronal specific Nbs1-deficient (Nbs1CNS-del) mouse model.Methods Microarray analysis was performed to identify differentially expressed lncRNAs between Nbs 1CNS-ctr and Nbs1CNS-del mice.Expression profiles of lncRNA Gm15577 and coding gene Negr1 in mice, primary cerebellar culture and cell lines were measured using RT-qPCR.Subcellular fractionation was performed to determine the subcellular localization of Gm15577.Results Gm15577 was specifically expressed in mice cerebellum in a developmentally regulated manner, which could be abolished upon Nbs 1-deficiency.Gm15577 was located in the intronic region of Negr1 in a reversed orientation.Gm15577 modulated the RNA expression of Negr1, Shh and β-catenin.NEGR1 had a distinct expression pattern between normal and medulloblastoma patients.Conclusion Gm15577 may modulate cerebellar granule cell proliferation and differentiation by targeting Negr 1, and their dysfunctions or abnormal expression may be related to tumorigenesis of medulloblastoma.
