Expression and function of ECRG4 in hepatocellular carcinoma
10.3760/cma.j.issn.0529-5807.2015.07.014
- VernacularTitle:食管癌相关基因4在肝细胞肝癌中的表达和功能
- Author:
Chen CHAO
1
;
Qian LAI
;
Taobo LUO
;
Gaoyan TANG
;
Ren ZHOU
;
Wei ZHANG
Author Information
1. 浙江大学医学院病理学与法医学研究所 浙江大学医学院病理学与病理生理学系
- Keywords:
Carcinoma,hepatocellular;
Cell proliferation;
Cell movement;
Apoptosis
- From:
Chinese Journal of Pathology
2015;(7):486-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of esophageal cancer related gene 4( ECRG4) in human hepatocellular carcinoma and the role of ECRG 4 in proliferation, apoptosis and migration of hepatoma cells .Methods ECRG4 expression was investigated in normal or tumor liver cell lines including QSG7701 and HepG2 cells, and in 24 pairs of fresh samples of hepatocellular carcinoma by quantitative real-time PCR or Western blot.ECRG4-pcDNA3.1 expressing plasmid was transfected into HepG 2 cells, of which cellular proliferation , apoptosis and migration were documented .Results ECRG4 mRNA expression was reduced or absent in most primary hepatocellular carcinoma samples ( 95.8%, 23 out of 24 hepatocellular carcinoma samples ) compared to their paired normal liver samples ( P <0.01 ) .ECRG4 mRNA was significantly lower in HepG2 cells than QSG7701 cells (P<0.05) along with decreased ECRG4 protein expression.HepG2 cells overexpressing ECRG 4 showed decreased proliferation , increased apoptosis and reduced migration as compared with control cells ( P <0.05 ) .Conclusions ECRG4 expression is frequently down-regulated in hepatocellular carcinoma.Overexpression of ECRG 4 inhibits the proliferation and migration but promotes apoptosis of HepG 2 cells,suggesting that ECRG4 is a candidate tumor suppressor gene in hepatocellular carcinoma and therefore may serve as a novel target for precision therapy .
