Inhibitory effect of salinomycin on human breast cancer cells MDA-MB-231 proliferation through Hedgehog signaling pathway
10.3760/cma.j.issn.0529-5807.2015.06.007
- VernacularTitle:盐霉素通过 Hedgehog 信号通路抑制乳腺癌细胞 MDA-MB-231的增殖
- Author:
Ying LU
1
;
Chunying ZHANG
;
Qing LI
;
Jun MAO
;
Wei MA
;
Xiaotang YU
;
Zhenhuan HOU
;
Lianhong LI
Author Information
1. 116044,大连医科大学辽宁省肿瘤干细胞研究重点实验室
- Keywords:
Hedgehog signaling pathway;
Cell proliferation
- From:
Chinese Journal of Pathology
2015;(6):395-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of salinomycin on human breast cancer cells in vitro, and to explore the related molecular mechanism.Methods Human breast cancer MDA-MB-231 cells were treated with salinomycin at different concentrations and at various time points.The effect of salinomycin on MDA-MB-231 cells proliferation was studied by CCK-8 method.The cell cycle status was examined by flow cytometry.RT-PCR and Western blot were used to detect the expression of Shh, Smo and Gli1 in the Hedgehog pathway at mRNA and protein levels.Results Proliferation of MDA-MB-231 cells treated with salinomycin was markedly inhibited in a concentration and time dependent manner.Salinomycin at concentrations of 0,0.4,0.8 and 1.6 μmol/L inhibited the growth at the rates of 11.18%,25.88%, 50.03%, 92.65%, respectively.Salinomycin prevented MDA-MB-231 cells from G1 into S phase.Salinomycin at concentrations of 0,0.8 and 1.6μmol/L resulted in S-phase percentage of 25.03%,11.85%and 35.21%, respectively ( P <0.05 ).RT-PCR and Western blot showed that the expression of key elements Shh, Smo and Gli1 in the Hedgehog pathway was inhibited by salinomycin in a concentration dependent manner( P<0.05) .Conclusion Salinomycin prevents breast cancer cell transition from G1 to S phase through downregulation of the target genes of Hedgehog signaling pathway, leading to an effective inhibition of MDA-MB-231 cells.
