Detection of p16 by fluorescence in-situ hybridization and immunohistochemistry in malignant mesothelioma
10.3760/cma.j.issn.0529-5807.2015.04.011
- VernacularTitle:荧光原位杂交与免疫组织化学检测 p16在恶性间皮瘤中的表达
- Author:
Ying LIU
1
;
Xiaoling CHEN
;
Ningning DING
;
Wenxiang XU
;
Hong YANG
;
Xuechen CHEN
Author Information
1. 110044,沈阳市胸科医院病理科
- Keywords:
Mesothelioma;
Genes,p16;
In situ hybridization,fluorescence;
Immunohistochemistry
- From:
Chinese Journal of Pathology
2015;(4):262-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of p16 gene mutation status as detected by fluorescence in-situ hybridization ( FISH) and p16 protein expression as detected by immunohistochemistry in differential diagnosis of malignant mesothelioma and benign mesothelial hyperplasia.Methods p16 gene mutation status and protein expression were detected by FISH and immunohistochemistry respectively in 55 cases of pleural malignant mesothelioma and 30 cases of benign mesothelial hyperplasia.Results FISH study showed that the rate of p16 deletion in malignant mesothelioma ( 81.8%,45/55 ) was higher than that in benign mesothelial hyperplasia ( 3.3%, 1/30 ) .The difference was statistically significant ( P <0.05 ) . Immunohistochemical study showed that the rate of p16 protein expression in malignant mesothelioma (23.6%) was lower than that in benign mesothelial hyperplasia ( 76.7%).The difference was also statistically significant.The sensitivity and specificity of FISH in distinguishing between mesothelioma and reactive mesothelial hyperplasia were higher than those of immunohistochemistry.Conclusions In contrast to reactive mesothelial hyperplasia, p16 gene is deleted and p16 protein is not expressed in malignant mesothelioma.The sensitivity and specificity of FISH are higher than those of immunohistochemistry in the distinction.
