Expression of microRNA-10a-5p in laryngeal epithelial premalignant lesions

VernacularTitle:microRNA－10a－5p在喉癌前病变组织中的表达
Author: Yanping HU ¹ ; Yulan JIN ; Xue LI ; Jia YU ; Fang WANG ; Wenjing BU ; Honggang LIU
Author Information

1. 首都医科大学潞河教学医院病理科
Keywords: Laryngeal neoplasms; Polymerase chain reaction; Pathology,clinical
From: Chinese Journal of Pathology 2015;(3):184-188
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of microRNA-10a-5p ( miR-10a) in laryngeal epithelial premalignant lesions ( LEPL ) and to analyze the correlations of its dys-regulation with clinicopathologic parameters of LEPL.Methods According to the WHO classification (2005), 94 cases of LEPL were grouped into mild dysplasia (MID), moderate dysplasia (MOD), severe dysplasia (SD) and carcinoma in situ (CIS).The expression of miR-10a in 94 cases of LEPL was detected by quantitative real-time polymerase chain reaction ( qRT-PCR) , and correlated with the clinical and follow-up data of all LEPL patients.Results miR-10a was down-regulated in LEPL with increasing grade of dysplasia.There was significantly statistical difference between low-risk ( MID+MOD) and high-risk ( SD+CIS) lesion groups ( P=0.03).The linear regression analysis showed that miR-10a was correlated with grade and gender of LEPL (P<0.05).Conclusions Down regulation of miR-10a may be a useful molecular marker for grading of LEPL and diagnosis of early laryngeal cancer.