Cannabinoid receptor 1 controls nerve growth in ectopic cyst in a rat endometriosis model

VernacularTitle:大麻素1型受体在大鼠子宫内膜异位病灶神经生长中的作用
Author: Qianqian ZHAO ¹ ; Xizi LIANG ; Hongxiu HAN
Author Information

1. 201999,上海交通大学医学院附属第三人民医院病理科
Keywords: Endometriosis; Nerve Fibers; Receptor,cannabinoid,CB1
From: Chinese Journal of Pathology 2014;(12):827-830
CountryChina
Language:Chinese
Abstract: Objective To investigate whether cannabinoid receptor 1 (CB1R) is involved in nerve growth in endometriosis-associated ectopic cyst.Methods The effect of CB1R agonist and antagonist on the expression of pan-neuronal marker protein gene product ( PGP ) 9.5 in ectopic cyst was examined by immunofluorescence and Western blot in endometriosis model of 18 rats.Results Immunofluorescence revealed that PGP 9.5 was expressed in the nerve fibers and was mainly distributed in the cyst hilum. Western blot revealed that the protein density of either PGP 9.5 (2 week:0.38 ±0.05;4 week:0.63 ± 0.03;8 week:0.80 ±0.07, P<0.01) or CB1R(2 week:0.48 ±0.04;4 week:0.68 ±0.01;8 week:0.80 ±0.03, P<0.01) in the ectopic cyst increased with cyst size.In addition, compared to control group (0.75 ±0.01), PGP 9.5 expression in the ectopic cyst was promoted by CB1R agonist ACPA (0.81 ± 0.01, P <0.05), and inhibited by CB1R antagonist AM251 (0.67 ±0.03, P <0.01) .Conclusions CB1R was involved in the nerve growth of ectopic cyst associated with endometriosis.