Correlation between BRAF V600E mutation and clinicopathologic features of papillary thyroid carcinoma
10.3760/cma.j.issn.0529-5807.2014.12.002
- VernacularTitle:BRAF V600E突变与甲状腺乳头状癌临床病理特征的关系
- Author:
Haizhen LU
1
;
Tian QIU
;
Jianming YING
;
Changyuan GUO
;
Ning LYN
Author Information
1. 中国医学科学院肿瘤医院病理科
- Keywords:
Thyroid neoplasms;
Pathology,clinical;
Prognosis
- From:
Chinese Journal of Pathology
2014;(12):794-798
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the prevalence of the BRAF V600E mutation in papillary thyroid carcinoma ( PTC) and its association with clinicopathologic features.Methods Two hundred and ninety-two patients with primary PTC encountered during the period from December 2010 to December 2012 and underwent surgery in Cancer Hospital, Chinese Academy of Medical Science were enrolled into the study. Polymerase chain reaction was used to amplify exon 15 of the BRAF gene from paraffin-embedded thyroid tumor specimens, followed by direct sequencing to detect the BRAF V600E mutation.Statistical analysis was performed with SPSS 16.0 for Windows. Association between BRAF mutation and clinicopathologic parameters was tested with theχ2 test or Fisher exact test as appropriate.Results There were 87 males and 205 females in the cohort. The age of patients ranged from 13 to 84 years ( mean =43.1 years ) . BRAF V600E mutation was found in 190 cases ( 65.1%) . The presence of BRAF V600E mutation correlated with age at diagnosis (older than 45 years), tumor volume (larger than 1 cm), extrathyroidal extension, classic type/tall-cell variant and advanced disease stage ( P<0.05).BRAF V600E mutation did not correlate significantly with gender, multicentricity, lymph node metastasis or anatomic location ( P >0.05).Conclusion BRAF V600E mutation is associated with high-risk clinicopathologic characteristics in patients with PTC.The BRAF V600E mutation may be a potential prognostic factor in PTC patients.
