Expression pattern of Mycobacterium tuberculosis Ag85B and its value in pathological diagnosis
10.3760/cma.j.issn.0529-5807.2014.09.006
- VernacularTitle:结核分枝杆菌Ag85B蛋白表达特点及其病理学诊断价值
- Author:
Nanying CHE
1
;
Yang QU
;
Chen ZHANG
;
Li ZHANG
;
Lijuan ZHOU
;
Dan SU
;
Yingli ZHAO
;
Chongli WANG
;
Haiqing ZHANG
Author Information
1. 101149,首都医科大学附属北京胸科医院 北京市结核病胸部肿瘤研究所病理科
- Keywords:
Tuberculosis;
Mycobacillin;
Immunohistochemistry;
Diagnosis,differential
- From:
Chinese Journal of Pathology
2014;(9):600-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of Mycobacterium tuberculosis secreted protein Ag85B in paraffin-embedded tissues by immunohistochemistry (IHC), and to evaluate its application in the pathological diagnosis of tuberculosis.Methods One hundred and five tuberculosis specimens (54 pulmonary tuberculosis, 51 lymph nodal tuberculosis ) and 51 specimens of other diseases (8 lung cancer, 10 pulmonary abscess, 10 bronchiectasis, 7 lymphoma, 5 necrotizing lymphadenitis, 4 reactive hyperplasia lymphoid , and 7 sarcoidosis ) were collected from January 2012 to July 2013 from Beijing Chest Hospital, Capital Medical University.One-step IHC was performed on paraffin-embedded tissues using antibody directed against Ag85B.Results IHC and Ziehl-Neelsen ( ZN) acid-fast staining showed that distribution and intensity of Ag85B expression were concordant with the distribution and number of acid-fast bacilli.IHC showed significantly higher sensitivity than ZN staining (50.5%,53/105 vs.31.4%,33/105;χ2 =7.877, P=0.005).The combined sensitivity of IHC and ZN staining was 59.0%.Moreover, oil immersion was not necessary for IHC , allowing more rapid diagnosis.Conclusion IHC detection of Ag85B is a simple method with higher sensitivity than ZN staining , and demonstrated good value in the pathological diagnosis of tuberculosis.
