Profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogen esis of rats induced by N, N'dinitrosopiperazine (DNP)
10.3760/j.issn:0529-5807.2001.02.011
- VernacularTitle:鼻咽上皮癌变过程中端粒酶活性和端粒酶RNA的表达
- Author:
Faqing TANG
1
;
Haiying JIANG
;
Zhaojun DUAN
;
Binglai CHEN
;
Zhaozheng JING
;
Shanghui WU
Author Information
1. Xiangya Hospital Hunan Medical University
- From:
Chinese Journal of Pathology
2001;30(2):125-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis (NPC) induced by N,N'dinitrosopi perazine (DNP) and examined histolofically. Methods Nasopharyngeal carcinomas o f rats were induced by DNP and examined histologically. PCR-ELISA and nested R T -PCR were used to assay telomerase and telomerase RNA expression at different s tages in the nasopharyngeal tissues of rats. Results During the carcinogenesis p rocess, telomerase activity increased along with the formation of a nasopharynge al carcinoma. Telomerase expression was positively related with nasopharyngeal c arcinogenesis. Telomerase RNA expression was present and did not change during t he NPC process. Expression of telomease RNA was earlier than telomerase activat ion. Telomerase activation and telomerase RNA expressin were also detected in th e pre-cancerous nasopharyngeal lesions. Conclusion Telomerase activation may p articipate in the onset and progression of NPC, and is an early step in NPC.
