The effects of sodium butyrate on proliferation, differentiation and apop tosis in immortalized esophageal epithelial cells
- VernacularTitle:丁酸钠对食管永生化上皮细胞增殖、分化和凋亡的作用
- Author:
Zhongying SHEN
1
;
Minghua CHEN
;
Weijia CAI
;
Jian SHEN
;
Jiongyu CHEN
;
Chaoqun HONG
;
Yi ZENG
Author Information
1. Shantou University Medical College
- From:
Chinese Journal of Pathology
2001;30(2):121-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of sodium butyrate on proliferatio n, differentiation and apoptosis of immortalized esophagus epithelial cells. Methods SHEE, an immortalized human fetal esophageal epithelial cell line induced by HPV18 E6E7, was cultivated in culture flasks and 24-well plates. Two experi m ent groups of cultured cells were treated with 1 and 5 mmol/L of sodium butyrate respectively for 4 days, and one group of untreated cells set aside as control. The numbers of cloned cells were calculated. The ultra-structure of SHEE cells was examined by transmission electron microscopy (TEM). The cell cycle and numbe r of apoptotic cells were measured by flow cytometry, Ki-67 and cytokeratin of ce lls were detected by immunohistochemistry method and F-actin of cells labeled w ith phalloidin was examined by laser confocal scanning microscopy. Results Colo ny formations showed a significant decrease in the 2 experiment groups after 4 d ays of culture (P<0.01). In the 1 mmol/L group, the cells at S phase were di minish ed and arrested at G0/G1 phase. Compared with control group, Ki-67 positive cells were found decreased, while F-actin and cyto keratin were increased. Apoptotic cells in 5 mmol/L group were increased markedl y. Conclusions Sodium butyrate may induce SHEE cells growth arrest,differentiation and apoptosis. The effects depend on sodium butyrate concentrat ion and time of exposure. Whether it can be used in combination with other antic ancer drugs should be further studied.
