Gene rearrangement and p53 expression in defining th e nature of angioimmunoblastic lymphadenopathy
10.3760/j.issn:0529-5807.2001.02.001
- VernacularTitle:基因重排分析与p53的表达在判定血管免疫母细胞性淋巴结病性质上的意义
- Author:
Peng ZHAO
1
;
Xiangrui JI
;
Hua ZHANG
;
Tianfu JIANG
;
Xianlu SUN
Author Information
1. The Hospital of Qingdao University Medical College
- From:
Chinese Journal of Pathology
2001;30(2):85-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate gene rearrangement and p53 expression i n defining the nature of angioimmunoblastic lymphadenopathy. Methods DNA was ext racted from paraffin-embedded tissue blocks of 44 angioimmunoblastic lymphaden o pathy (AIL) patients and analyzed with polymerase chain reaction (PCR) for IgH and TCRγ gene rearrangement. Immunohistochemistry staining was used to detect p53 protein expression. Thirty-five cases were followed-up. Results 12 out of 44 cases(27.3%) showed TCRγ gene rearr angement and 2 (4.5%) showed IgH gene rearrangement. Rearrangement of both IgH a nd TCRγ genes were detected in 2 cases(4.5%). 14 cases (31.8%) showed p53 posit i ve expression, among which 12 showed positive rearrangement and 2 showed negative (P<0.01). Eight out of 11 patients of p os itive gene rearrangement died in one year, while only 3 patients were still aliv e at the eighteenth month of follow-up, three of 24 patients of negative gene r earrangement were found dead at the time of the one year follow-up, while the r est 21 patients were alive and the longest survival time was 96 months. Conclusions Gene rearrangement can define th e pathological nature of AIL. The expression of p53 is highly related to gene r earrangement, and thus an important immunological marker in research on AIL.
