A quantitative pathological study on angiogenesis, vascular endothelial growth factor and inducible nitric oxide synthase in astrocytomas
10.3760/j.issn:0529-5807.2001.01.007
- VernacularTitle:星形细胞肿瘤血管生成活性和血管内皮生长因子与诱生型一氧化氮合酶表达的定量研究
- Author:
Xiuwu BIAN
1
;
Linlin DU
;
Ziqiang CHEN
;
Jingquan SHI
;
Fengxuan LIU
Author Information
1. Southwestern Hospital Third Military Medical University
- From:
Chinese Journal of Pathology
2001;30(1):23-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether the expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) are asscoiated with angiogenesis and malignancy of astrocytomas. Methods Immunohistochemistry, semiquantitation and image analysis were used to study the expression features of VEGF and iNOS in 61 cases of astrocytomas having follow-up data. Factor Ⅷ related antigen (FⅧ RAg) was also analysed in reflecting the epithelial cell amount and the microvessel density. Results Microvessels in astrocytomas could be divided into seven types. The positive reaction areas and IA values of FⅧRAg expression in the endothelium were significantly increased with the increase of tumor grade （P<0.001）and VEGF labeling index (LI) （P<0.05）. The patients with high VEGF LIs （LI≥25%） had a poor prognosis. VEGF expression was significantly related to that of iNOS（P<0.001), i.e., VEGF level was reduced along with the decrease of iNOS reaction. Conclusion Detection of FⅧRAg could reflect the angiogenesis activities in astrocytomas. VEGF and iNOS could up-regulate each other to promote angiogenesis and are of importance in determining the malignancy degree of astrocytomas.
