Detection of methylation status of p16 tumor suppressor gene in soft tissue leiomyosarcoma
10.3760/j.issn:0529-5807.2001.01.005
- VernacularTitle:软组织平滑肌肉瘤中p16基因的甲基化检测
- Author:
Jianmin XU
1
;
Guanghua YANG
;
Hong BU
;
Lixin GUO
;
Shiyu ZHANG
;
Ji LIU
Author Information
1. 中山大学
- From:
Chinese Journal of Pathology
2001;30(1):16-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the methylation status of p16 tumor suppressor gene in human soft tissue leiomyosarcoma (LMS) and its relationship with p16 protein expression. Methods Methylation-specific PCR (MSP) assay was used to analyze the methylation status of 5′CpG island promoter / exon I region of p16 tumor suppressor gene in 38 LMS, 10 leiomyomas and 5 normal smooth muscle tissues. p16 expression in these tissues were examined by immunohistochemical assay. Results Hypermethylation of p16 was observed in 23.7% (9/38) of cases, of which 7 with negative pl6 staining and 2 with faint positive staining. In the cases without protein expression, hypermethylation rate of p16 was 50% (7/14). Conclusions Epigenetic change due to 5′CpG methylation is the main cause of inactivation of p16(INK4) gene which may be involved in tumor pathogenesis.
