Impact of Carcinoma-associated Fibroblasts on Biological Behavior of Androgen-independent Prostate Cancer Cells
10.3969/j.issn.1001-1420.2009.07.023
- VernacularTitle:癌相关成纤维细胞对雄激素非依赖性前列腺癌细胞生物学行为的影响
- Author:
PAN WENHAI
1
;
HUA XING
Author Information
1. 广东省广州市红十字会医院
- Keywords:
prostate cancer;
cancer-associated fibroblasts;
infiltration;
metastasis
- From:
Journal of Clinical Urology
2009;24(7):549-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of cancer-associated fibroblasts(CAFs) in the microenviron-ment of prostate cancer on biological behavior of androgen-independent prostate cancer cell line Du-145. Methods: Prostate cancer CAFs was primary cultured and CAFs conditioned medium(CAFs-FM) was produced. Through the proliferation experiment, flat colony-forming experiments and invasion assay to observe the impact of CAFs on the biological behavior of prostate cancer cells. Results: CAFs isolated from fresh human prostate cancer tissue and CAFs-CM dose-dependent increased the Du-145 cell's proliferation capacity. Compared with SFM, 0.5 g/L CAFs-CM significantly enhanced the Du-145 cell's proliferation capacity, both there was a significant difference (290.6± 25.2)vs(88.4±12.1) (P<0.05), Du-145 cell proliferation index Ki-67 was correspongingly significantly im-proved (80% vs 20%). Compared with cultured in DMEM, flat panel capacity of colony-forming and the move-ment capacity of deformation were markedly improved, there were significant differences(0.42±0.09)vs(0.23± 0.03)(P<0.05) and(162.12±9.13)vs(112.34±8.45)(P<0.01) respectively. Conclusions:CAFs in the pros-tate cancer stromal microenvironment promote prostate cancer cell invasion and metastasis. CAFs is a new target for drug design in the course of the treatment of prostate cancer.
