Induction of apoptosis by all trans-retinoic acid in bladder tumor T24 cell line
10.3969/j.issn.1001-1420.2001.02.010
- VernacularTitle:全反式维甲酸诱导膀胱癌T24细胞株凋亡的研究
- Author:
Xuejun ZHANG
1
;
Linglong WANG
;
Shixing YANG
Author Information
1. Xiangfan City Central Hospital
- From:
Journal of Clinical Urology
2001;16(2):75-76
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To explore the all-trans-retinoic acid(ATRA) on induction of apoptosis in human bladder transitional cell carcinoma cell line T24.Methods: The apoptosis of T24 cell line was studied by fluorescence microscopy, flow cytometry and DNA agarose gel electrophoresis.Results:After T24 cells were treated by 3×10-6 mol/L ATRA six days, the apoptotic rates of T24 cells were 15.20% in treated group and 0.01% in control group respectively under fluorescence microscopy(P<0.05); the apoptotic rates of T24 cells were 15.31% in treated group and 1.49% in control group separately on flow cytometry ; a characteristic DNA “ladder” on agarose gels was observed in treated group.Conclusions:The apoptosis of human bladder transitional cell carcinoma cell line T24 can be induced by ATRA.
