Expression of induce nitric oxide synthase in experimental cryptorchidism
10.3969/j.issn.1001-1420.2000.10.016
- VernacularTitle:诱生型一氧化氮合成酶基因在隐睾中的表达及意义
- Author:
ZHENG-hang
1
;
Xin-Ming ZHENG
;
Shi-Wen LI
;
Li-Qian HU
;
Xiao-Peng LI
Author Information
1. Second Affiliated Hospital, Hubei Medical University
- Keywords:
Cryptorchism Nitric oxide synthase Germ cells Cells apoptosis
- From:
Journal of Clinical Urology
2000;15(10):471-472
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To study the expression of induced nitric oxide synthase(iNOS) and testicular germ cellapoptosis in experimental cryptorchidism. Methods: Immature rats (22 day-old, Sprague Dawley) were subjectedto unilateral cryptorchidism; Using terminal-deoxynucleotidy1 transferase mediated dUTP nick end labeling(TUNEL)for the detection of germ cell apoptosis; Using immunohistochemical SP method for the detection ofexpression of iNOS. Results:①After operation, the number of apoptotic germ cells in the cryptorchidism tissueincreased when the time prolonged within 7 days. ②The number of apoptotic germ cells in the cryptorchidism tis-sue increased significantly as compared to the contralateral control testis of the same animal from the 4th day tothe 7th day after operation.③In control testis, iNOS immunostaining was Localized to the cytoplasm of Leydigcell, sertoli cells and primary spermatocytes; Additional intense immunostaining was detected in the cytoplasm ofsloughed germ cells of tubules in the cryptorchidism. Conclusions:Expression of iNOS is believed to be one of thecauses that increase the number of apoptotic germ cells in the cryptorchidism.
