mRNA expression of costimulatory molecular B7 in rat renal tissue after warm ischemia/reperfusion injury

VernacularTitle:共刺激分子B7 mRNA在大鼠热缺血再灌注损伤肾组织中的表达
Author: Shi-Lin ZHANG ¹ ; Zhi-Lian MIN ; You-Hua ZHU
Author Information

1. the Changzheng Hospital Second Military Medical University of PLA
Keywords: B7 Kidney transplantation Reperfusion injury Rats; inbred strains Polymerase chain reac-tion Kidney
From: Journal of Clinical Urology 2000;15(10):469-470
CountryChina
Language:Chinese
Abstract: Purpose:To further testify the role of costimulatory molecular B7 in renal ischemia/reperfusion in-jury. Methods:The mRNA transcription level of B7 was assayed with reverse transcription semiquantative poly-merase chain reaction in the uninephrectomized rat model established by warm ischemia/reperfusion. Results :ThemRNA transcription level of B7 was very low in normal and ischemia renal tissue, but was gradually increased af-ter repefusion,and reached peak level at 72 hours after reperfusion; The mRNA transription level of B7 in the is-chemia 60 min reperfusion group was higher than that in the ischemia 30 min reperfusion group. Conclusions :Up-regulation of costimulatory molecular B7 further elucidates the mechanism of relationship between ischemia/reperfusion injury and acute rejection; More heavier ischemia/reperfusion injury, more possible acute rejection.