Analysis for epitope polymorphism of class HLA-Ⅰ antigen from regular platelet donors in Nanjing area
10.13602/j.cnki.jcls.2024.10.04
- VernacularTitle:南京地区血小板定期捐献者HLA-Ⅰ类抗原表位多态性分析
- Author:
Yu ZHANG
1
;
Chengtao HE
;
Jie CAI
;
Lei LV
;
Lu ZHANG
;
Xiaolu HE
;
Hailin DU
;
Qiang FU
;
Chun ZHANG
Author Information
1. 南京红十字血液中心研究室,南京 210003
- Keywords:
platelet transfusion refractoriness;
human leukocyte antigen;
epitope;
polymorphism
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(10):738-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of epitope distribution on HLA class Ⅰ antigen in the regular platelet donors from Nanjing area and establish the HLA epitope database for regular platelet donors.Methods High-resolution HLA typing was per-formed using Sanger method for the blood samples from 649 regular platelet donors in Nanjing area.The polymorphism of HLA antigen epitopes corresponding to the high-resolution HLA typing results was analyzed using the HLA Eplet Registry website.The frequencies of allele frequencies,HLA haplotype,and HLA antigen epitope were calculated by using the direct counting method.Results Among the 649 regular platelet donors,38 HLA-A alleles were detected,corresponding to 36 HLA-A epitopes,and the higher frequencies were 79GT,144K and 138MI.Seventy-three HLA-B alleles were detected corresponding to 35 HLA-B epitopes,and the higher frequencies were 131S,69TNT,and 80N.Sixty-four HLA class Ⅰ antigen epitopes were detected,in which the higher frequencies were 79GT,131S,and 144K.Conclusions The distribution of HLA antigen epitopes in the regular platelet donors of Nanjing area exhibited u-nique polymorphic characteristics.The epitope matching strategies should be established based on the distribution characteristics of HLA antigen epitopes,which may expand the range of available donors and reduce the incidence of platelet transfusion refractoriness.
