Expression and clinical significance of miRNA identified by high-throughput sequencing in left ventricular hypertrophy
10.13602/j.cnki.jcls.2024.09.12
- VernacularTitle:基于高通量测序分析miRNA在左心室心肌肥厚中的表达及意义
- Author:
Kaiyu ZHANG
1
;
Yuan LI
;
Jingyi XU
;
Buyun QIAN
;
Kangyun SUN
;
Feng WANG
Author Information
1. 南京医科大学附属苏州医院&苏州市立医院心血管病中心,江苏苏州 215008
- Keywords:
miRNA;
left ventricular hypertrophy;
high-throughput sequencing;
diagnosis
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(9):695-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels and clinical significance of differentially expressed microRNAs(miRNAs)based on high-throughput sequencing in patients with left ventricular hypertrophy(LVH)and their possible regulatory mechanisms.Methods The miRNA sequencing was performed on plasma samples from 4 LVH patients and 4 healthy volunteers,and qRT-PCR validation was performed on plasma samples from 25 healthy volunteers and 35 LVH patients.The target genes of the obtained differentially expressed miRNA were predicted.The molecular function(MF),biological process(BP)and cellular components(CC)of these miRNAs were predicted by the Gene Ontology(GO)enrichment analysis,and their signal pathways were predicted by the Kyoto Encyclopedia of Genes and Genomes analysis.Meanwhile,the protein-protein interaction network was constructed.The diagnostic values of 2 differentially expressed miRNAs(hsa-miR-942-5p and hsa-miR-184)in LVH were analyzed by the area under the receiver operating characteristic(ROC)curve(AUCROC).Results A total of 945 differentially expressed miRNAs were identified,of which 1 and 9 were observed to be significantly upregulated and downregulated,respectively.The expression levels of hsa-miR-942-5p and hsa-miR-184 were verified by qRT-PCR to be significantly downregulated.The AUCROC of hsa-miR-942-5p and hsa-miR-184 were 0.694 6 and 0.880 4,respectively.Bioinformatics analysis revealed that the target genes were mainly enriched in the cell senescence,sphingolipid metabolism,and TGF-β,p53,and diabetic metabolism related signaling pathways.Conclusion The expression levels of hsa-miR-942-5p and hsa-miR-184 in plasma of LVH patients are significantly decreased,suggesting that they have good diagnostic potential.
