Investigation on the mechanism of EZH2 regulating biological behavior of ovarian cancer cells
10.13602/j.cnki.jcls.2024.07.13
- VernacularTitle:EZH2调控卵巢癌细胞生物学行为的机制研究
- Author:
Ling ZHU
1
,
2
;
Ke WANG
;
Feng ZHAO
;
Siqi LI
;
Shukui WANG
;
Qixiang SHAO
Author Information
1. 南京医科大学附属南京医院检验科,南京 210006
2. 江苏护理职业学院江苏省消化及生殖系统肿瘤精准防治工程研究中心医学遗传与生殖免疫研究所,江苏淮安 223005
- Keywords:
ovarian cancer;
histone methyltransferase;
enhancer of zeste homolog 2;
early growth response 1
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(7):542-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of enhancer of zeste homolog 2(EZH2),a histone methyltransferase,in regula-ting the biological behavior of ovarian cancer(OC)and provide the experimental support for finding new therapeutic targets in the treat-ment of OC.Methods The small interfered RNAs(siRNAs)of EZH2 were used to knock down EZH2 in different OC cell lines,and the interfering efficiency of siEZH2 mRNAs and protein were evaluated by qRT-PCR and Western blot.The cell proliferation,migra-tion,invasion ability and apoptosis of OC cells before and after EZH2 interference were evaluated by the CCK-8,wound healing,Tran-swell and flow cytometry.The expression levels of early growth response 1(EGR1)and H3K27me3 proteins after EZH2 interference were determined by Western blot.Meanwhile,the mechanism of EZH2 regulating the biological behavior of OC cells was explored.Re-sults The expression levels of EZH2 mRNA in OC cells transfected with siEZH2 were significantly lower than that in the negative con-trol group(P＜0.05)and the expression levels of EZH2 protein in A2780 cells were also significantly downregulated(P＜0.05).The results of Western blot showed that the expression levels of EGR1 and H3K27me3 proteins were reduced to varying degrees.After trans-fection with siEZH2-1,the proliferation ability of A2780 cells in the transfected group was significantly lower than that in the negative control group(P＜0.05).The results of the cell scratch test and Transwell test showed that the migration and invasion ability of OC cells transfected with siEZH2-1 were significantly weakened(P＜0.05).The results of flow cytometry showed that the apoptosis of OC cells transfected with siEZH2-1 was significantly enhanced(P＜0.05).Conclusion EZH2 is highly expressed in OC cells and can promote the proliferation,migration,invasion and anti-apoptosis of A2780 cells.However,EZH2 affects the biological behavior of ovar-ian cancer not by regulating the expression of EGR1 through its H3K27me3 transferase activity.
