Clinical significance of the detection of SORT1 gene in peripheral blood mononuclear cells for the diagnosis of pediatric sepsis
10.13602/j.cnki.jcls.2024.07.03
- VernacularTitle:外周血单个核细胞SORT1基因检测在小儿脓毒血症诊断中的临床意义
- Author:
Yan LI
1
;
Jinchao WANG
;
Genhao ZHANG
Author Information
1. 安阳市人民医院输血科,河南安阳 455000
- Keywords:
pediatric sepsis;
sortilin 1 gene;
receiver operating characteristic curve;
gene set enrichment analysis
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(7):492-496
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance of sortilin 1(SORT1)gene as a new diagnostic biomarker for pediatric sep-sis.Methods Three pediatric sepsis-related microarray datasets,including GSE13904,GSE26378,and GSE26440,were obtained from the gene expression omnibus(GEO)database to analyze the expression difference of SORT1 gene between normal and sepsis sam-ples.Then,65 clinical samples were used to validate the difference through qRT-PCR.The molecular mechanism and biological process associated with SORT1 gene were explored by the gene set enrichment analysis(GSEA).Results The expression levels of SORT1 gene in the sepsis samples of GSE13904,GSE26378,and GSE26440 datasets and clinical samples were higher than that in normal samples.Compared with commonly used inflammatory markers such as procalcitonin(PCT),C-reactive protein(CRP),white blood cell(WBC),and the percentage of neutrophils(Neu％),SORT1 gene showed better diagnostic ability for pediatric sepsis and the area under the receiver operating characteristic(ROC)curve(AUCROC)was 0.863.SORT1 gene may be involved in the occurrence and de-velopment of pediatric sepsis through the TNF-α/NF-κB signaling pathway.Conclusion SORT1 gene may have certain diagnostic val-ue for pediatric sepsis,which needs to be further confirmed in clinical practice.
