Evaluation of concordance between liquid chromatography-tandem mass spectrometry and fully automated chemiluminescence immunoassay for determination of plasma aldosterone concentration
10.13602/j.cnki.jcls.2024.06.02
- VernacularTitle:液相色谱-串联质谱法和全自动化学发光免疫法测定血浆醛固酮浓度的一致性评价
- Author:
Chunjing YANG
1
;
Xiqiao XU
;
Li BAO
;
Zhengyuan SHI
Author Information
1. 首都医科大学附属北京世纪坛医院药剂科,临床合理用药评价北京市重点实验室,临床合理用药评价国际合作联合实验室,北京 100038
- Keywords:
elderly patients;
aldosterone;
liquid chromatography-tandem mass spectrometry;
chemiluminescent immunoassay;
myoglobin
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(6):406-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the consistency of liquid chromatography-tandem mass spectrometry(LC-MS/MS)and fully auto-mated chemiluminescent immunoassay(CLIA)methods in measuring plasma aldosterone concentration(PAC)in the elderly patients in intensive care unit(ICU)and to explore the correlation between the levels of aldosterone(ALD)and blood biomarkers.Methods A total of 41 elderly ICU patients were included.PAC was measured using both LC-MS/MS and CLIA methods,followed by methodolo-gy validation and consistency comparison.Meanwhile,a retrospective analysis of the patients'clinical data was conducted,and the cor-relations between ALD and blood biomarkers were analyzed.Results The linear range of the LC-MS/MS method was 15 to 1 500 pg/mL,with a lower limit of quantification of 15 pg/mL.The inter-day and intra-day precisions were both less than 15％,and the re-covery rate was 94％to 99％,with no significant matrix effect.The PAC results detected by CLIA and LC-MS/MS methods were posi-tively correlated(r=0.762 7,P＜0.01),but the consistency between the two methods was poor.Deming regression analysis yielded the equation Y=0.969 6X-16.71,with a slope of 0.970(95％CI:0.890～1.049)and an intercept of-16.71(95％CI:-25.690 to-7.728).Bland-Altman analysis showed that CLIA overestimated PAC by an average of 21.18 pg/mL(95％CI:-25.89 to 68.26 pg/mL)compared to LC-MS/MS,with a bias of 24.02％.In the correlation analysis between ALD and blood biomarkers,ALD showed a significant positive correlation with myoglobin(Mb)(r=0.303,P＜0.05).Conclusion The LC-MS/MS method demonstrated good methodological performance in measuring PAC in the elderly patients in ICU.The consistency between LC-MS/MS and CLIA methods was poor,and the two methods should not be used interchangeably in clinical practice.There was a positive correlation between ALD and Mb,suggesting that ALD may be associated with myocardial injury.
