Characteristics and homology analysis of drug sensitivity of Escherichia coli mediated by the mobile polymyxin resistance gene
10.13602/j.cnki.jcls.2024.05.02
- VernacularTitle:移动性多黏菌素耐药基因介导的大肠埃希菌药敏特点与同源性分析
- Author:
Shulong ZHAO
1
;
Shuang SONG
;
Le LIU
;
Jingjing XU
;
Yinhai XU
;
Haiquan KANG
Author Information
1. 徐州医科大学附属医院检验科,江苏徐州 221002
- Keywords:
Escherichia coli;
mobile polymyxin resistance gene;
pulsed field gel electrophoresis;
resistance;
homology analysis
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(5):327-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the resistance mechanism,characteristics of drug sensitivity,and homology of polymyxin resistant Escherichia coli,and provide molecular epidemiological basis for the effective prevention and control of its outbreak and epidemic.Methods The strains of polymyxin resistant Escherichia coli isolated from our hospital during May 2016 and June 2022 were collected.The PCR technology was used to screen for the mobile colistin resistance(MCR)gene and the micro broth dilution method was used to determine the minimum inhibitory concentration.The clinical data of the patients were collected.The homology of the collected strains was analyzed by the pulsed field gel electrophoresis(PFGE).One of the strains was performed whole genome sequencing.Results Four strains of polymyxin resistant Escherichia coli carrying the MCR-1 gene were isolated and identified.Except for being sensitive to tigecycline,all four strains showed varying degrees of resistance to the vast majority of clinically common antibiotics.Clinical data showed that no polymyxin was used in the patients with polymyxin resistant Escherichia coli.The four strains of bacteria could be divided into two types,including three strains of type A and one strain of type B.The whole genome sequencing analysis of one of the bacteria revealed that the MCR-1 gene was located on a plasmid with a size of 70 kb.Conclusion The Escherichia coli carrying the MCR-1 gene has a potential threat to clone transmission and exhibits high resistance to common antibiotics in clinical practice,posing a serious challenge to clinical treatment.Therefore,it is necessary to strengthen protective measures to prevent its outbreak and epidemic.
