Characterization of Escherichia coli lytic phage vB_EcoP-R1 and its anti-biofilm activity
10.13602/j.cnki.jcls.2024.04.05
- VernacularTitle:大肠埃希菌裂解性噬菌体vB_EcoP-R1的特性及其抗细菌生物膜活性分析
- Author:
Zhixin SHI
1
,
2
;
Zexuan LI
;
Litao ZHANG
;
Xin HONG
;
Jing LIU
;
Zhe ZHAO
;
Genyan LIU
Author Information
1. 南京医科大学第一附属医院检验学部,南京 210029
2. 南京医科大学第一附属医院医学检验国家临床重点专科,南京 210029
- Keywords:
Escherichia coli;
bacteriophage;
biological characteristics;
phage therapy;
biofilm
- From:
Chinese Journal of Clinical Laboratory Science
2024;42(4):261-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate E.scherichia coli-specific bacteriophages from hospital waste water,analyze their biological character-istics and detect their cleavage effects on bacterial biofilms.Methods The phages were isolated and purified by double-layer agar plate method and their morphology was observed by transmission electron microscopy.Their one-step growth curve,in vitro lysis curve,tem-perature and acid-base stability were determined.Whole genome sequencing was used to analyze the genome composition and character-istics of bacteriophages.The crystal violet staining was used to evaluate the scavenging effects of phage on host biofilms.Results A novel Escherichia coli-specific bacteriophage named vB_EcoP-R1 was isolated from hospital sewage.Transmission electron microscopy revealed that the morphology of vB_EcoP-R1 was similar to that of Podoviridae,a member of Caudovirales,and it could survive stably in the temperature range of-20 ℃ to 50 ℃ and pH 5.0 to 11.0.The total length of the vB_EcoP-R1 genome was 40 875 bp and the GC content was 49.94％.There were no virulence genes and drug resistance genes in the phage genome.vB_EcoP-R1 was capable of lysing clinically isolated Escherichia coli and showed high species and genus specificity.After the phage acted on the host bacteria preformed biofilm for 4 h,the clearance rate of the biofilm reached 80.16％.Conclusion vB_EcoP-R1 should be a novel Escherichia coli-specif-ic bacteriophage with strong lysis and high specificity for species and genus.The bacteriophage could lyse the biofilm formed by the host bacteria,and is expected to be used as an alternative treatment for Escherichia coli infection.
