Neutrophil extracellular traps regulating the development and prognosis of oral squamous cell carcinomas
10.13591/j.cnki.kqyx.2024.07.004
- VernacularTitle:中性粒细胞胞外诱捕网调控口腔鳞状细胞癌的发展及预后的相关研究
- Author:
Jie YI
1
;
Yujie DING
;
Yufei SHAN
;
Jiaqi GU
;
Zhida SUN
Author Information
1. 南京医科大学附属口腔医院牙体牙髓科,口腔疾病研究与防治国家级重点实验室培育建设点(南京医科大学),江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Keywords:
neutrophil extracellular traps;
oral squamous cell carcinoma;
epithelial-mesenchymal transition
- From:
STOMATOLOGY
2024;44(7):500-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and role of neutrophil extracellular traps(NETs)in oral squamous cell carcinoma(OSCC).Methods Immunofluorescence and immunohistochemistry were used to detect the expression of NETs in OSCC.The clinico-pathological characteristics and prognosis of patients with different NETs expression levels were analyzed.Density gradient centrifugation was used to isolate neutrophils from human peripheral blood.NETs were induced by phorbol 12-myristate 13-acetate(PMA)and co-cultured with OSCC cells.CCK-8 assay was used to detect changes in OSCC cell proliferation ability.OSCC cell migration ability was detected by Transwell cell migration assay and cell scratch assay.Western blotting(WB)assay was used to detect the effect of NETs on the index of epithelial-mesenchymal transition(EMT).Results NETs'expression in OSCC was higher than that in normal tissues(P＜0.001).The prognosis of patients with high NETs expression was worse than that of patients with low NETs expression(P＜0.05).The expression level of NETs was correlated with the clinical grade,invasion and recurrence degree of OSCC patients(P＜0.05).NETs pro-moted the proliferation and migration of Cal27 and HN6 cells(P＜0.05),and inhibited the protein expression level of epithelial marker and promoted the protein expression level of mesenchymal markers(P＜0.05),which could be reversed by the NETs inhibitor DNaseⅠ.Conclusion NETs are expressed at high levels in OSCC.NETs can promote the proliferation and migration of OSCC cells by regulating epithelial-mesenchymal transition and can affect the prognosis of OSCC patients.
