Effects of quercetin loaded gelatin microspheres on proliferation and differentiation of MC3T3-E1
10.13591/j.cnki.kqyx.2024.07.003
- VernacularTitle:载槲皮素明胶微球对MC3T3-E1增殖和分化的影响
- Author:
Weijie DONG
1
;
Tingshu SU
;
Xianzhen XIN
Author Information
1. 嘉兴市第一医院口腔科,浙江嘉兴(314000)
- Keywords:
gelatin;
quercetin;
microspheres;
osteogenic differentiation;
bone tissue engineering;
mouse embryonic osteoblast pre-cursor cells(MC3T3-E1)
- From:
STOMATOLOGY
2024;44(7):494-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study prepared gelatin three-dimensional porous microspheres and investigated the feasibility of gelatin three-dimensional porous microspheres loaded with quercetin(G-quercetin)as bone tissue scaffold material.Methods Porous gelatin microspheres were prepared by emulsification and loaded with quercetin by lyophilization.Scanning electron microscopy was used to ob-serve morphology of the microspheres.The cytotoxicity of G-quercetin microspheres and their effects on the adhesion,proliferation and differentiation of mouse embryonic osteoblast precursor cells(MC3T3-E1)were detected by immunofluorescence staining,live/dead cell staining and CCK-8 assay,alkaline phosphatase(ALP)staining and alizarin red staining.RT-PCR was used to detect the tran-scriptional levels of osteoblast-related cytokines such as Runx-2,ALP,OPN and OCN.Results The scanning electron microscopy re-sults showed that the prepared three-dimensional microporous material loaded with quercetin gelatin had a porous structure.Cell adhe-sion showed that the cells could spread well on the surface of the microspheres.Compared with the control group,the results of live/dead cell staining and CCK-8 detection showed that the microspheres had no significant cytotoxicity(P＞0.05).Compared with the con-trol group,G-quercetin microspheres showed an increase in ALP expression and mineralization in vitro.PCR results also showed a sig-nificant increase in Runx-2,ALP,OCN,OPN(P＜0.05).Conclusion The G-quercetin porous microspheres prepared in this experi-ment have good biocompatibility and can promote the osteogenic differentiation of MC3T3-E1 in vitro.It is expected to be used as a new scaffold material for bone tissue engineering.
