The effect of NF-κB signaling pathway regulation on PD-L1 expression of Enterococcus faecalis LTA-stimulated macrophages
10.13591/j.cnki.kqyx.2024.07.001
- VernacularTitle:NF-κB信号通路调控对粪肠球菌脂磷壁酸刺激下巨噬细胞PD-L1表达的影响
- Author:
Yanling YANG
1
;
Runze LIU
;
Qing SUN
;
Wei FAN
Author Information
1. 武汉大学口腔医学院口颌系统重建和再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医(学)院,湖北武汉(430079)
- Keywords:
Enterococcus faecalis;
lipoteichoic acid;
immune checkpoint;
NF-κB
- From:
STOMATOLOGY
2024;44(7):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Enterococcus faecalis(E.faecalis)lipoteichoic acid(LTA)on the expression of the macrophage immune checkpoint-programmed cell death protein ligand 1(PD-L1)and explore the possible regulatory mechanisms.Methods In vitro model was established by stimulating mouse monocyte macrophage cell line(RAW264.7)with different concentra-tions of E.faecalis LTA.PD-L1 expression and nuclear factor kappa-B(NF-κB)p65 phosphorylation level were detected using flow cy-tometry,quantitative real-time polymerase chain reaction(qRT-PCR),Western blot and cellular immunofluorescence.Macrophages were pretreated with the NF-κB signaling pathway inhibitor BAY 11-7082,then the expression of PD-L1 and p65 phosphorylation level were detected by the above methods.Results LTA induced PD-L1 expression in RAW264.7 cells,and p65 phosphorylation level in-creased(P＜0.05).Inhibition of the NF-κB signaling pathway could reduce p65 phosphorylation level,simultaneously reducing the ex-pression of PD-L1 on LTA-stimulated RAW264.7 cells(P＜0.05).Conclusion E.faecalis LTA can promote the PD-L1 expression of macrophages and this process is regulated by the NF-κB signaling pathway,which provides further insights into the occurrence and treatment of chronic periapical inflammation.
