Study on the antibacterial performance and biocompatibility of silver nanoparticals-coated root canal nickel titanium instruments
10.13591/j.cnki.kqyx.2024.06.007
- VernacularTitle:银纳米粒子涂层根管镍钛器械的抗菌性能及生物相容性研究
- Author:
Hong JIN
1
;
Huiwen WANG
;
Yuting WU
;
Mingrui DAI
;
Diya LENG
;
Tingting ZHU
;
Daming WU
Author Information
1. 南京医科大学附属口腔医院牙体牙髓病科,口腔疾病研究与防治国家级重点实验室培育建设点(南京医科大学),江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Keywords:
root canal nickel titanium instrument;
silver nanoparticles(AgNPs)-coated;
antibacterial performance;
biocompatibility
- From:
STOMATOLOGY
2024;44(6):438-442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antibacterial performance and biocompatibility of silver nanoparticles-coated root canal nickel titanium instruments(AgNPs-NiTi).Methods AgNPs-NiTi was prepared using pulse electrochemical deposition.The morphol-ogy of AgNPs-NiTi was observed using field emission scanning electron microscopy(FE-SEM),and the elemental composition and con-tent were analyzed using X-ray diffraction(XRD)and energy dispersive spectroscopy(EDS).The mechanical properties of AgNPs-NiTi were tested.After Co-culturing AgNPs-NiTi with E.faecalis,the antibacterial effect was detected by colony-forming units method.By constructing an in vitro model of E.faecalis biofilm in the root canal of teeth,the antibacterial effect of AgNPs-NiTi was observed using FE-SEM and live/dead bacterial staining.In addition,AgNPs-NiTi was co-cultured with Raw 264.7 cells,and its cytotoxicity was de-tected by CCK-8.Results The pulse electrochemical deposition was used to construct a silver nanoparticle(AgNPs)coating on NiTi instruments with no significant change in the mechanical properties.AgNPs-NiTi significantly inhibited the proliferation of E.faecalis and damaged E.faecalis biofilm in the root canal.AgNPs-NiTi had no significant influence on the proliferation of Raw264.7 cells and had no cytotoxicity.Conclusion The mechanical properties of AgNPs-NiTi are similar to those of nickel titanium instruments.AgNPs-NiTi inhibits E.faecalis proliferation with good biocompatibility.
