Effects of Versican V0/V1 gene silencing on biological behaviors of mouse dental papilla cells
10.13591/j.cnki.kqyx.2024.05.006
- VernacularTitle:沉默Versican V0/V1对小鼠牙乳头细胞生物学行为的影响
- Author:
Jiayan WU
1
;
Haiyan HUANG
;
Chenyu SONG
;
Beizhan JIANG
Author Information
1. 同济大学口腔医学院·附属口腔医院儿童口腔科,上海牙组织修复与再生工程技术研究中心,上海(200072)
- Keywords:
Versican;
mouse dental papilla cell;
cell proliferation;
cell migration;
odontogenic differentiation
- From:
STOMATOLOGY
2024;44(5):349-355
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Versican V0/V1 on biological behaviors of mouse dental papilla cells(mDPCs).Methods mDPCs were isolated from C57BL/6J mice at embryonic day 16.5(E16.5).A small interfering RNA(siRNA)constructed specifically for Versican V0/V1 was transfected into mDPCs.The silencing efficiency was verified by quantitative real-time fluorescence polymerase chain reaction(qRT-PCR)and immunofluorescence staining.The proliferation rate of mDPCs was determined using EdU assay;the migration ability of mDPCs was evaluated by scratch test and transwell assay.Alkaline phosphatase(ALP)staining and aliz-arin red staining were used to assess the mineralization capability of mDPCs.The molecules related to odontogenic differentiation and mineralization at mRNA levels were measured by qRT-PCR.Results After siRNA transfection,the mDPCs of si-Versican V0/V1 group showed weaker proliferation and migration abilities compared with si-NC group(P＜0.01).An enhanced ALP staining intensity,miner-alized nodule formation and up-regulations of the molecules related to odontogenic differentiation and mineralization at mRNA levels(P＜0.05)were observed in the mDPCs of si-Versican V0/V1 group.Conclusion Versican V0/V1 silencing inhibits the proliferation and migration of mDPCs,but enhances the abilities of odontogenic differentiation and mineralization.
