Mechanism of molecular hydrogen attenuating acute lung injury induced by lipopolysaccharid
10.3760/cma.j.issn.1671-0282.2024.10.011
- VernacularTitle:分子氢减轻脂多糖诱导急性肺损伤的机制探究
- Author:
Haoyue XUE
1
;
Xinyi TANG
;
Jinqiu DING
;
Xiaobing CHEN
;
Haoran CHEN
;
Dian YU
;
Xiaomin LI
;
Yongpeng XIE
Author Information
1. 徐州医科大学附属连云港医院连云港市第一人民医院急诊科，连云港 222006
- Keywords:
Acute lung injury;
Ferroptosis;
Molecular hydrogen;
Alveolar epithelial cells;
Lipopolysaccharide;
Nuclear factor erythroid 2-related factor;
Glutathione pe
- From:
Chinese Journal of Emergency Medicine
2024;33(10):1413-1420
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and mechanism of molecular hydrogen in lipopolysaccharide (LPS)-induced acute lung injury (ALI).Methods:Balb/c male mice were randomly(random number) divided into control group, control+H 2, LPS and LPS+H 2 group with 6 mice in each group. The levels of malondialdehyde (MDA) and Fe 2+ in lung tissue were detected by kits. The lung tissue morphology was observed. The infiltration levels of F4/80 positive macrophages in lung tissue were detected by immunofluorescence staining. A549 cells were divided into control, control+H 2, erastin and erastin+H 2 group. The reactive oxygen species (ROS), malondialdehyde, (MDA), lactate dehydrogenase (GSH), number of cell death and lactate dehydrogenase (LDH) release in each group were detected by kits. Nrf2, GPX4, and HO-1mRNA were quantified by real-time PCR, the protein expression level of Nrf2 was detected by western blot, and the nuclear translocation level of Nrf2 was observed by immunofluorescence. The chi-square test was performed before the measurement data were counted. One-way analysis of variance was used to compare differences between multiple groups. Results:Compared with the control group, the histopathological damage was aggravated, and the levels of MDA, Fe 2+ significantly increased in the LPS group, and F4/80 positive immune cells infiltration significantly increased (all P<0.05). Compared with LPS group, the degree of lung injury in LPS+H 2 group significantly reduced (all P<0.05). In vitro experiments, compared with the control group, the ROS, MDA levels, number of cell death and LDH release significantly increased in erastin group (all P<0.05), while GSH, and GPX4 mRNA levels decreased (all P<0.05). HO-1mRNA and Nrf2 nuclear translocation levels increased (all P<0.05). Compared with erastin group, ROS, MDA levels, cell death number and LDH release decreased in earstin+H 2 group (all P<0.05). The levels of GSH, GPX4 mRNA, Nrf2 mRNA, HO-1 mRNA and Nrf2 nuclear translocation levels increased (all P<0.05). Conclusions:Molecular hydrogen attenuates LPS-induced ALI by promoting Nrf2 nuclear translocation to inhibit ferroptosis of alveolar epithelial cells.
