Expression of miR-34a and TNF-α in neonatal rat model of acute respiratory distress syndrome lung injury
10.3760/cma.j.issn.1671-0282.2024.06.006
- VernacularTitle:miR-34a与TNF- α在新生大鼠急性呼吸窘迫综合征肺损伤模型中的表达
- Author:
Xiu WANG
1
;
Jie ZHANG
;
Xiaoli WANG
;
Mengyue HUO
;
Chun XIN
;
Hua MEI
Author Information
1. 内蒙古医科大学附属医院新生儿科，呼和浩特 010050
- Keywords:
Neonatal acute respiratory distress syndrome;
miRNA-34a;
TNF-α;
Neonates
- From:
Chinese Journal of Emergency Medicine
2024;33(6):767-773
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of microRNA-34a (miR-34a)and tumor necrosis factor-α (TNF-α) in lung injury induced by lipopolysaccharides (LPS) in neonatal rats with acute respiratory distress syndrome (ARDS). The expression of mirna-34a and tumor necrosis factor-α (TNF-α) in lung injury induced by lipopolysaccharides (LPS) was determined by tumor necrosis factor.Methods:A total of 80 7-day-old newborn SD rats were randomly(random number) divided into two groups, 40 in each group, NARDS animal models were established by intraperitoneal injection of 4 mg/kg LPS solution for 3,6,12 and 24 hours respectively. Normal control group was established by intraperitoneal injection of 4 mL/kg isotonic Nacl solution. The wet/dry weight ratio of lung was measured to observe the changes of lung histopathology. The expression of miR-34a in lung tissues and cells was detected by quantitative real-time PCR (qrt-PCR).Results:the wet/dry weight of lung in the experimental group was significantly higher than that in the control group. Hematoxylin-eosin (HE) staining showed diffuse changes in lung tissue, thickening of alveolar septum, partial alveolar fusion and decreased number of alveoli in experimental group. The expression levels of miR-34a and TNF-α in the experimental group were higher than those in the control group at each time point.Conclusions:It is speculated that miR-34a can promote the release of more inflammatory factors by positively regulating the expression of TNF-α, thus affecting the occurrence and development of lung injury. The expression of miR-34a positively correlated with TNF-α. miR-34a may affect the regulation mechanism of lung injury by mediating the expression of TNF-α through some signal pathway or inflammatory reaction, it provides a new therapeutic target for the treatment of neonatal acute respiratory distress syndrome (NARDS).
