Etiological diagnostic value of metagenomic Next Generation Sequencing for bloodstream infection in patients with sepsis
10.3760/cma.j.issn.1671-0282.2024.04.008
- VernacularTitle:宏基因组二代测序对脓毒症患者血流感染的病原学诊断价值
- Author:
Yunyu YIN
1
;
Xuefeng DING
;
Daqing WANG
;
Ying GE
;
Li CHEN
Author Information
1. 川北医学院附属医院重症医学科，南充 637000
- Keywords:
mNGS;
Blood culture;
Sepsis;
Etiological diagnosis
- From:
Chinese Journal of Emergency Medicine
2024;33(4):497-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the value of metagenomic Next Generation Sequencing (mNGS) for the pathogenetic diagnosis of bloodstream infections in patients with sepsis.Methods:A retrospective analysis was performed on 105 sepsis cases who received blood mNGS and blood culture tests during their hospitalization in the Intensive Care Department of the Affiliated Hospital of North Sichuan Medical College from September 2021 to August 2023, and the results were compared and analyzed. According to mNGS and blood culture results, the cases were divided into the positive group and negative group.The distribution of pathogens in the enrolled patients were analyzed, and the diagnostic performance and consistency of blood culture and mNGS were compared. The differences in clinical characteristics of patients in each group were analyzed, and the pathogenic diagnostic value of mNGS for bloodstream infections in patients with sepsis was evaluated.Results:①Among 105 blood samples, 61 cases (58.10%) had positive results of mNGS, and 32 cases (30.48%) had positive results of blood culture. The positive rate of mNGS was higher than that of blood culture, and the difference was statistically significant ( P < 0.05).The accuracy of mNGS was lower than that of blood culture②The mean values of ESR, PCT and CRP in positive group were higher than those in negative group, and the proportion of septic shock was higher than that in negative group, and the differences were statistically significant ( P < 0.05). There was no significant difference in 28-day mortality rate between the two groups ( P > 0.05). Conclusions:mNGS is beneficial to the etiological diagnosis and treatment of bloodstream infection in patients with sepsis.
