Quality evaluation of ultrafine powder of Spatholobus suberectus based on HPLC fingerprint combined with stoichiometry methods
10.7644/j.issn.1674-9960.2024.11.005
- VernacularTitle:基于HPLC指纹图谱结合化学计量学方法分析评价鸡血藤超微粉的质量
- Author:
Guoxue WU
1
;
Shuting YANG
;
Dong LIU
;
Xiaoxia LI
;
Jing DONG
;
Yibin HAO
;
Yishuo WANG
Author Information
1. 河南中医药大学第五临床医学院/郑州人民医院,郑州 450003
- Keywords:
Spatholobus suberectus;
ultrafine powder;
fingerprint;
cluster analysis;
principal component analysis;
least partial squares-discriminant analysis
- From:
Military Medical Sciences
2024;48(11):832-837
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality of ultrafine powder of Spatholobus suberectus from different sources by combined HPLC fingerprint and chemical pattern recognition.Methods Separation was performed on a Amethyst C18-H column(4.6 mmx250 mm,5 pm)and the mobile phase was 0.1％phosphoric acid-acetonitrile with gradient elution.The flow rate was 1.0 mL/min and the wavelength was 260 nm.The column temperature was 30 ℃.The cluster analysis(CA),least partial squares-discriminant analysis(PLS-DA)and principal component analysis(PCA)were applied to studying the HPLC fingerprint and chemical pattern recognition.Results Thirteen common peaks were identified in ultrafine powder samples of 10 batches of Spatholobus suberectus and 3 components were identified by reference substances.The similarity of fingerprint was 0.921-0.989,indicating good similarity.The samples were divided into 3 groups by cluster analysis;principal component analysis results extracted 4 principal components.According to the composite score,the quality of ultrafine powder samples of Spatholobus suberectus from S6,S1,S2,S3 were better than others.And 4 kinds of marker compounds that caused the quality difference of ultrafine powder samples of Spatholobus suberectu were screened out through the least partial squares-discriminant analysis,which were epicatechin and peaks 1,6 and 4 respectively.Conclusion The fingerprint method is simple and reproducible,and can be used to evaluate the quality of ultrafine powder samples of Spatholobus suberectus in combination with multi-mode stoichiometric analysis.
